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KMID : 0613820190290040461
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Journal of Life Science
2019 Volume.29 No. 4 p.461 ~ p.469
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Effect of Ethanolic Extracts Mixed with Grains and Fallopia multiflora on Melanogenesis
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Lee Eun-Been
Kim Moon-Moo
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Abstract
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The aim of this study was to investigate the effect of a mixture of ethanol extracts of Black oryzasativa, Sesamum indicum, Oryza sativa, Rhynchosia Nulubilis, and Polygoni multiflori radix (MIXEE) on melanogenesis to develop a natural product for black hair growth. An accumulation of hydrogen peroxide (H2O2) in hair follicles, which reduces melanin synthesis, is responsible for hair graying. In the present study, MIXEE showed scavenging activity against DPPH radicals and reducing power. In addition, it reduced the cellular H2O2 level, indicating that it could inhibit oxidation and promote melanin synthesis, which was decreased by H2O2. On the other hand, it did not affect tyrosinase activity in vitro but promoted the turnover of L-DOPA into melanin. MIXEE promoted melanin synthesis at the cellular level in B16F1 cells. Furthermore, MIXEE increased the expression levels of superoxide dismutase 2 (SOD2) and SOD3 in western blot analysis. In addition, MIXEE increased the expression levels of tyrosinase-related protein (TRP)-2, which promoted melanin synthesis from L-DOPA. The results suggested that MIXEE could promote melanogenesis. Therefore, MIXEE may have potential as a natural product for promotion of melanin production and reversal of gray hair to black hair.
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KEYWORD
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Black oryzasativa, Oryza sativa, Polygoni multiflori radix, Rhynchosia Nulubilis, Sesamum indicum
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